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normal complement-preserved human serum  (BioIVT Inc)

 
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    BioIVT Inc normal complement-preserved human serum
    Normal Complement Preserved Human Serum, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+complement-preserved+human+serum/normal+complement+preserved+human+serum/pmc06331245-110-9-11
    Average 90 stars, based on 1 article reviews
    normal complement-preserved human serum - by Bioz Stars, 2026-10
    90/100 stars

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    Incubation:

    Article Title: The human complement receptor type 2 (CR2)/CR1 fusion protein TT32, a novel targeted inhibitor of the classical and alternative pathway C3 convertases, prevents arthritis in active immunization and passive transfer mouse models.
    Article Snippet: These kits were used to assess the effect of TT32, TT30, sCR1 (R&D Systems), CR1 1–10 (Alexion) and anti-CR2 mAbs (clones 171, 629 (Alexion) and 1048 (Green Mountain)) on each pathway, according to manufacturers' instructions. .. TT32 and other compounds were diluted in normal complement-preserved human serum (Bioreclamation, Westbury, NY) and incubated in presence of AP/CP pathway-specific buffer diluent at 37 °C. ..

    Article Title: The Human Complement Receptor Type 2 (CR2)/CR1 Fusion Protein TT32, a Novel Targeted Inhibitor of the Classical and Alternative Pathway C3 Convertases, Prevents Arthritis in Active Immunization and Passive Transfer Mouse Models
    Article Snippet: These kits were used to assess the effect of TT32, TT30, sCR1 (R&D Systems), CR1 1–10 (Alexion) and anti-CR2 mAbs (clones 171, 629 (Alexion) and 1048 (Green Mountain)) on each pathway, according to manufacturers’ instructions. .. TT32 and other compounds were diluted in normal complement-preserved human serum (Bioreclamation, Westbury, NY) and incubated in presence of AP/CP pathway-specific buffer diluent at 37°C. ..



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    SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different complement pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% NHS containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.

    Journal: Blood Advances

    Article Title: SAR443809: a selective inhibitor of the complement alternative pathway, targeting complement factor Bb

    doi: 10.1182/bloodadvances.2022009028

    Figure Lengend Snippet: SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different complement pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% NHS containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.

    Article Snippet: Complement preserved normal human serum (NHS) was sourced from Complement Technology Inc (Tyler, TX), BioIVT (Westbury, NY), or Innovative Research (Novi, MI).

    Techniques: Control, Inhibition, Activation Assay, Hemolysis Assay, Incubation, Standard Deviation